Hamburg was one of five urban areas left in the running, close by Rome, Paris, Budapest and Los Angeles.
However, 51.7% of occupants of the city and close-by Kiel, where cruising occasions would have occurred, voted no in a choice on Sunday.
German Olympics authorities had picked Hamburg as their favored applicant city in front of Berlin.
Hamburg's chairman Olaf Scholz said: "That is a choice that we didn't need, yet it's unmistakable."
Germany has not facilitated the Games subsequent to 1972 in Munich.
Faultfinders of the arrangement said it was inefficient, at an expense of €11.2bn ($11.9bn; £7.9bn), just a little division of which had been guaranteed by city powers.
A representative for the International Olympic Committee (IOC) said that "an incredible open door for the city, the nation and game in Germany is lost".
"The city likewise misses the speculation of the IOC of about $1.7bn to the achievement of the Games, which analyzes to the €1.2bn Hamburg needed to contribute," he included.
"Presently there will be a solid rivalry with four brilliant hopeful urban areas. With these solid contenders we all can anticipate [an] energizing Olympic Games 2024, whoever the victor will be."
Two years prior, the general population of Munich likewise voted against the Olympics, turning down the opportunity to have the 2022 Winter Games.
In July, the city of Boston chose to pull back from the race to be host for 2024 in the midst of an absence of open backing.
The triumphant city will be chosen in September
